Recombinant Human PSMC5 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human PSMC5 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : PSMC5-6105H
Product Overview : PSMC5 MS Standard C13 and N15-labeled recombinant protein (NP_002796) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. In addition to participation in proteasome functions, this subunit may participate in transcriptional regulation since it has been shown to interact with the thyroid hormone receptor and retinoid X receptor-alpha. Two transcript variants encoding different isoforms have been found for this gene.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 45.6 kDa
AA Sequence : MALDGPEQME LEEGKAGSGL RQYYLSKIEE LQLIVNDKSQ NLRRLQAQRN ELNAKVRLLR EELQLLQEQG SYVGEVVRAM DKKKVLVKVH PEGKFVVDVD KNIDINDVTP NCRVALRNDS YTLHKILPNK VDPLVSLMMV EKVPDSTYEM IGGLDKQIKE IKEVIELPVK HPELFEALGI AQPKGVLLYG PPGTGKTLLA RAVAHHTDCT FIRVSGSELV QKFIGEGARM VRELFVMARE HAPSIIFMDE IDSIGSSRLE GGSGGDSEVQ RTMLELLNQL DGFEATKNIK VIMATNRIDI LDSALLRPGR IDRKIEFPPP NEEARLDILK IHSRKMNLTR GINLRKIAEL MPGASGAEVK GVCTEAGMYA LRERRVHVTQ EDFEMAVAKV MQKDSEKNMS IKKLWKTRTR PLEQKLISEE DLAANDILDY KDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : PSMC5 proteasome 26S subunit, ATPase 5 [ Homo sapiens (human) ]
Official Symbol : PSMC5
Synonyms : PSMC5; proteasome (prosome, macropain) 26S subunit, ATPase, 5; 26S protease regulatory subunit 8; p45; p45/SUG; S8; SUG 1; SUG1; TBP10; TRIP1; MSUG1 protein; proteasome subunit p45; thyroid receptor interactor 1; Tat-binding protein homolog 10; proteasome 26S ATPase subunit 5; proteasome 26S subunit ATPase 5; 26S proteasome AAA-ATPase subunit RPT6; thyroid hormone receptor-interacting protein 1; SUG-1;
Gene ID : 5705
mRNA Refseq : NM_002805
Protein Refseq : NP_002796
MIM : 601681
UniProt ID : P62195

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