Recombinant Human PSMF1 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human PSMF1 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : PSMF1-3701H
Product Overview : PSMF1 MS Standard C13 and N15-labeled recombinant protein (NP_848693) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 29.8 kDa
AA Sequence : MAGLEVLFAS AAPAITCRQD ALVCFLHWEV VTHGYCGLGV GDQPGPNDKK SELLPAGWNN NKDLYVLRYE YKDGSRKLLV KAITVESSMI LNVLEYGSQQ VADLTLNLDD YIDAEHLGDF HRTYKNSEEL RSRIVSGIIT PIHEQWEKAN VSSPHREFPP ATAREVDPLR IPPHHPHTSR QPPWCDPLGP FVVGGEDLDP FGPRRGGMIV DPLRSGFPRA LIDPSSGLPN RLPPGAVPPG ARFDPFGPIG TSPPGPNPDH LPPPGYDDMY LTRTRPLEQK LISEEDLAAN DILDYKDDDD KV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : PSMF1 proteasome inhibitor subunit 1 [ Homo sapiens (human) ]
Official Symbol : PSMF1
Synonyms : PSMF1; proteasome (prosome, macropain) inhibitor subunit 1 (PI31); proteasome inhibitor PI31 subunit; PI31; proteasome inhibitor hP131 subunit; hPI31;
Gene ID : 9491
mRNA Refseq : NM_178578
Protein Refseq : NP_848693
MIM : 617858
UniProt ID : Q92530

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