Recombinant Human PTGS2 Protein, His-tagged PTGS2-63H

Recombinant Human PTGS2 Protein, His-tagged


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Recombinant Human PTGS2 Protein, His-tagged

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Cat.No. : PTGS2-63H
Product Overview : Recombinant human PTGS2(20-604) protein with a N-terminal His-tag was expressed in insect cells.
Description : Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.
Source : Insect cells
Species : Human
Tag : His
Molecular Mass : 67.9 kDa
Protein length : 20-604
Purity : ≥75% estimated by SDS-PAGE
Unit Definition : One unit of enzyme consumes one nanomole of oxygen per minute at 37 centigrade in 0.1 M Tris-HCl buffer, pH 8.0, containing 100 μM arachidonate, 5 mM EDTA, 2 mM phenol, and 1 μM hematin. The cyclooxygenase activity of COX-2 was measured at 37 centigrade by monitoring oxygen consumption using a Gilson Model 5/6 H oxygraph equipped with a Clark oxygen electrode.
Stability : ≥ 1 year
Storage : Store it at -80 centigrade
Storage Buffer : 80 mM Tris, pH 8.0, with 0.3 mM DDC, 0.01% Tween 20, and 10% glycerol
SDS-PAGE Analysis of COX-2 :

Lane 1: MW Markers; Lane 2: COX-2 (2 μg); Lane 3: COX-2 (5 μg); Lane 4: COX-2 (10 μg).Representative gel image shown; actual purity may vary between each batch.

Gene Name : PTGS2 prostaglandin-endoperoxide synthase 2 [ Homo sapiens (human) ]
Official Symbol : PTGS2
Synonyms : PTGS2; prostaglandin-endoperoxide synthase 2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS; prostaglandin G/H synthase 2; PGH synthase 2; PHS II; cyclooxygenase 2; cyclooxygenase 2b; prostaglandin H2 synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
Gene ID : 5743
mRNA Refseq : NM_000963
Protein Refseq : NP_000954
MIM : 600262
UniProt ID : P35354

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