Recombinant Human PTPN1

• Cat.No.: PTPN1-537H
• Product Overview: Recombinant Human protein tyrosine phosphatase, non-receptor type 1 was expressed inE.coliand it was purified by conventional column chromatographic techniques. MW = 37.3 kDa (321 aa).

Specification

• Cat. No.: PTPN1-537H
• Description: The protein coding region of the catalytic domain of PTP-1B (amino acids 1-321) was cloned into an E. coli expression vector. The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues.
• Source: Escherichia Coli.
• Form: Liquid. In 25 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT 2 mM β-mercaptoethanol, 1 mM EDTA, 20%Glycerol.
• Purity: > 95% by SDS - PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MEMEKEFEQI DKSGSWAAIY QDIRHEASDF PCRVAKLPKN KNRNRYRDVS PF D HSRIKLH QEDNDYINAS LIKMEEAQRS YILTQGPLPNTCGHFWEMVW EQKS R G V VML NRVMEKGSLK CAQYWPQKEE KEMIFEDTNL KLTLISEDIK SYYT VRQLEL ENLTTQETREILHFHYTTWPDFGVPESPAS FLNFLFKVRE SGS LSPE HGPV VV H CSAGIG RSGTFCLADT CLLLMDKRKD PSSVDIKKVLLEMRKFRMGLIQTADQL RFS YLAVIEGAKF IMGDSSVQDQ WKELSHEDLE PPPEHIPPPP RPP KR IL E PH N .
• Specific Acitivity: 10,000units/mg of PTP1B.
• Unit Definition: One unit will hydrolyze 1nanomole of p-nitrophenyl phosphatate per minute at pH 7.4 at 37°C using 10mM of substrate.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PTPN1 protein tyrosine phosphatase, non-receptor type 1 [ Homo sapiens ]
• Synonyms: PTPN1; protein tyrosine phosphatase, non-receptor type 1; Tyrosine-protein phosphatase non-receptor type 1; EC 3.1.3.48; Protein-tyrosine phosphatase 1B; PTP-1B; PTP1B; OTTHUMP00000031266; protein tyrosine phosphatase 1B; Protein-tyrosine phosphatase 1B; protein tyrosine phosphatase, placental
• Gene ID: 5770
• mRNA Refseq: NM_002827
• Protein Refseq: NP_002818
• MIM: 176885
• UniProt ID: P18031
• Chromosome Location: 20q13.1-q13.2
• Pathway: Adherens junction; Insulin signaling pathway; Hemostasis; Integrin cell surface interactions
• Function: hydrolase activity; insulin receptor binding; protein binding; protein tyrosine phosphatase activity; zinc ion binding