Recombinant Human PUF60 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human PUF60 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : PUF60-4007H
Product Overview : PUF60 MS Standard C13 and N15-labeled recombinant protein (NP_510965) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 59.7 kDa
AA Sequence : MATATIALVN GQQGGGSEPA AAAAVVAAGD KWKPPQGTDS IKMENGQSTA AKLGLPPLTP EQQEALQKAK KYAMEQSIKS VLVKQTIAHQ QQQLTNLQMA AVTMGFGDPL SPLQSMAAQR QRALAIMCRV YVGSIYYELG EDTIRQAFAP FGPIKSIDMS WDSVTMKHKG FAFVEYEVPE AAQLALEQMN SVMLGGRNIK VGRPSNIGQA QPIIDQLAEE ARAFNRIYVA SVHQDLSDDD IKSVFEAFGK IKSCTLARDP TTGKHKGYGF IEYEKAQSSQ DAVSSMNLFD LGGQYLRVGK AVTPPMPLLT PATPGGLPPA AAVAAAAATA KITAQEAVAG AAVLGTLGTP GLVSPALTLA QPLGTLPQAV MAAQAPGVIT GVTPARPPIP VTIPSVGVVN PILASPPTLG LLEPKKEKEE EELFPESERP EMLSEQEHMS ISGSSARHMV MQKLLRKQES TVMVLRNMVD PKDIDDDLEG EVTEECGKFG AVNRVIIYQE KQGEEEDAEI IVKIFVEFSI ASETHKAIQA LNGRWFAGRK VVAEVYDQER FDNSDLSATR TRPLEQKLIS EEDLAANDIL DYKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : PUF60 poly-U binding splicing factor 60KDa [ Homo sapiens (human) ]
Official Symbol : PUF60
Synonyms : PUF60; poly-U binding splicing factor 60KDa; poly(U)-binding-splicing factor PUF60; FBP interacting repressor; FIR; pyrimidine tract binding splicing factor; Ro ribonucleoprotein binding protein 1; RoBPI; siah binding protein 1; SIAHBP1; roBP1; siah-BP1; ro-binding protein 1; siah-binding protein 1; FBP-interacting repressor; poly-U binding splicing factor PUF60; 60 kDa poly(U)-binding-splicing factor; Ro ribonucleoprotein-binding protein 1; fuse-binding protein-interacting repressor; FLJ31379;
Gene ID : 22827
mRNA Refseq : NM_078480
Protein Refseq : NP_510965
MIM : 604819
UniProt ID : Q9UHX1

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