Recombinant Human PUS1 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human PUS1 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : PUS1-6139H
Product Overview : PUS1 MS Standard C13 and N15-labeled recombinant protein (NP_079491) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : This gene encodes a pseudouridine synthase that converts uridine to pseudouridine once it has been incorporated into an RNA molecule. The encoded enzyme may play an essential role in tRNA function and in stabilizing the secondary and tertiary structure of many RNAs. A mutation in this gene has been linked to mitochondrial myopathy and sideroblastic anemia. Alternate splicing results in multiple transcript variants.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 47.5 kDa
AA Sequence : MGLQLRALLG AFGRWTLRLG PRPSCSPRMA GNAEPPPAGA ACPQDRRSCS GRAGGDRVWE DGEHPAKKLK SGGDEERREK PPKRKIVLLM AYSGKGYHGM QRNVGSSQFK TIEDDLVSAL VRSGCIPENH GEDMRKMSFQ RCARTDKGVS AAGQVVSLKV WLIDDILEKI NSHLPSHIRI LGLKRVTGGF NSKNRCDART YCYLLPTFAF AHKDRDVQDE TYRLSAETLQ QVNRLLACYK GTHNFHNFTS QKGPQDPSAC RYILEMYCEE PFVREGLEFA VIRVKGQSFM MHQIRKMVGL VVAIVKGYAP ESVLERSWGT EKVDVPKAPG LGLVLERVHF EKYNQRFGND GLHEPLDWAQ EEGKVAAFKE EHIYPTIIGT ERDERSMAQW LSTLPIHNFS ATALTAGGTG AKVPSPLEGS EGDGDTDTRT RPLEQKLISE EDLAANDILD YKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : PUS1 pseudouridylate synthase 1 [ Homo sapiens (human) ]
Official Symbol : PUS1
Synonyms : PUS1; pseudouridylate synthase 1; tRNA pseudouridine synthase A, mitochondrial; tRNA uridine isomerase I; tRNA-uridine isomerase I; tRNA pseudouridylate synthase I; tRNA pseudouridine(38-40) synthase; mitochondrial tRNA pseudouridine synthase A; MLASA1; MGC11268;
Gene ID : 80324
mRNA Refseq : NM_025215
Protein Refseq : NP_079491
MIM : 608109
UniProt ID : Q9Y606

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