Recombinant Human PUS3 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: PUS3-4633H
• Product Overview: PUS3 MS Standard C13 and N15-labeled recombinant protein (NP_112597) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene catalyzes the formation of tRNA pseudouridine from tRNA uridine at position 39 in the anticodon stem and loop of transfer RNAs. Two transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 55.6 kDa
• AA Sequence: MADNDTDRNQTEKLLKRVRELEQEV QRLKKEQAKNKEDSNIRENSSGAGK TKRAFDFSAHGRRHVALRIAYMGWG YQGFASQENTNNTIEEKLFEALTKT RLVESRQTSNYHRCGRTDKGVSAFG QVISLDLRSQFPRGRDSEDFNVKEE ANAAAEEIRYTHILNRVLPPDIRIL AWAPVEPSFSARFSCLERTYRYFFP RADLDIVTMDYAAQKYVGTHDFRNL CKMDVANGVINFQRTILSAQVQLVG QSPGEGRWQEPFQLCQFEVTGQAFL YHQVRCMMAILFLIGQGMEKPEIID ELLNIEKNPQKPQYSMAVEFPLVLY DCKFENVKWIYDQEAQEFNITHLQQ LWANHAVKTHMLYSMLQGLDTVPVP CGIGPKMDGMTEWGNVKPSVIKQTS AFVEGVKMRTYKPLMDRPKCQGLES RIQHFVRRGRIEHPHLFHEEETKAK RDCNDTLEEDNTNLETPTKRVCVDT EIKSIITRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PUS3 pseudouridylate synthase 3 [ Homo sapiens (human) ]
• Official Symbol: PUS3
• Synonyms: PUS3; pseudouridylate synthase 3; MRT55; FKSG32; NEDMIGS; 2610020J05Rik; tRNA pseudouridine(38/39) synthase; pseudouridylate synthase 3; tRNA pseudouridine synthase 3; tRNA pseudouridylate synthase 3; tRNA-uridine isomerase 3; EC 5.4.99.45
• Gene ID: 83480
• mRNA Refseq: NM_031307
• Protein Refseq: NP_112597
• MIM: 616283
• UniProt ID: Q9BZE2