Active Recombinant Human PVR Protein, Fc & Avi-tagged, Biotinylated

• Cat.No.: PVR-653H
• Product Overview: Biotinylated Active Recombinant Human PVR Protein(P15151-1)(Trp21-Asn343) is expressed from HEK293 with hFc (IgG1) tag and Avi tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: 21-343 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Biotinylated Human CD155, hFc Tag at 5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Human TIGIT, His Tag with the EC50 of 1.14μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 62.82 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: PVR poliovirus receptor [ Homo sapiens ]
• Official Symbol: PVR
• Synonyms: PVR; poliovirus receptor; PVS; CD155; HVED; Necl 5; NECL5; nectin like 5; Tage4; nectin-like 5; nectin-like protein 5; TAGE4; Necl-5; FLJ25946
• Gene ID: 5817
• mRNA Refseq: NM_001135768
• Protein Refseq: NP_001129240
• MIM: 173850
• UniProt ID: P15151