Recombinant Human PVR Protein, hFc-tagged, Alexa Fluor 647 conjugated

• Cat.No.: PVR-620HAF647
• Product Overview: Alexa Fluor 647 conjugated recombinant human PVR (Trp21-Asn343) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells.
  CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells. The fulllength (or CD155 alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20 aa signal sequence, a 323 aa extracellular region, a 24 aa TM segment and a 50 aa cytoplasmic tail. The extracellular region contains one N terminal V type and two C2 type Ig like domains.
  CD155 is a transmembrane protein with 3 extracellular immunoglobulin-like domains, D1-D3, where D1 is recognized by the virus. Low resolution structures of CD155 complexed with poliovirus have been obtained using electron microscopy while a high resolution structures of theectodomain D1 and D2 of CD155 were solved by x-ray crystallography.
• Source: HEK293
• Species: Human
• Tag: hFc
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 61.7 kDa. The protein migrates as 95-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Trp21-Asn343
• N-terminal Sequence Analysis: Trp 21
• Endotoxin: < 1.0 EU/ μg by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: PVR
• Official Symbol: PVR
• Synonyms: PVR; poliovirus receptor; PVS; CD155; HVED; Necl 5; NECL5; nectin like 5; Tage4; nectin-like 5; nectin-like protein 5; TAGE4; Necl-5; FLJ25946
• Gene ID: 5817
• mRNA Refseq: NM_001135768
• Protein Refseq: NP_001129240
• MIM: 173850
• UniProt ID: P15151