Active Recombinant Human PVRIG protein, mFc-tagged, Low Endotoxin

• Cat.No.: PVRIG-390H
• Product Overview: Recombinant Human PVRIG protein(Q6DKI7)(Thr41-Asp171), fused with C-terminal mFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: mFc
• Protein Length: 41-171 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Nectin-2, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 92.1ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 40.03 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: PVRIG poliovirus receptor related immunoglobulin domain containing [ Homo sapiens ]
• Official Symbol: PVRIG
• Synonyms: PVRIG; poliovirus receptor related immunoglobulin domain containing; transmembrane protein PVRIG; C7orf15; MGC2463; poliovirus receptor-related immunoglobulin domain-containing protein; MGC104322; MGC138295; MGC138297
• Gene ID: 79037
• mRNA Refseq: NM_024070
• Protein Refseq: NP_076975
• UniProt ID: Q6DKI7