Recombinant Human RAB10 protein, His-tagged RAB10-7264H

Recombinant Human RAB10 protein, His-tagged

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Recombinant Human RAB10 protein, His-tagged

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Cat.No. : RAB10-7264H
Product Overview : Recombinant Human RAB10 protein(NP_057215.3)(1-196 aa) was fused to His-tagged and expressed in E. coli.
Description : The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane within the polarized neuron and epithelial cells. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane while in epithelial cells, it regulates transport from the Golgi to the basolateral membrane. Moreover, may play a role in the basolateral recycling pathway and in phagosome maturation.may play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion.
Source : E. coli
Species : Human
Tag : His
Form : Tris-based buffer,50% glycerol
Molecular Mass : 26.1kDa
Protein length : Partial
AA Sequence : MAKKTYDLLFKLLLI GDSGVGKTCVLFRFS DDAFNTTFISTIGID FKIKTVELQGKKIKL QIWDTAGQERFHTIT TSYYRGAMGIMLVYD ITNGKSFENISKWLR NIDEHANEDVERMLL GNKCDMDDKRVVPKG KGEQIAREHGIRFFE TSAKANINIEKAFLT LAEDILRKTPVKEPN SENVDISSGGGVTGW K
Purity : Greater than 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
SDS-PAGE of RAB10-7264H :

Gene Name : RAB10
Official Symbol : RAB10
Synonyms : GTP binding protein RAB10; Rab10; RAB10 member RAS oncogene family; RAB10_HUMAN; Ras related GTP binding protein; Ras related GTP binding protein RAB10; Ras-related protein Rab-10
Gene ID : 10890
mRNA Refseq : NM_016131.5
Protein Refseq : NP_057215.3
MIM : 612672
UniProt ID : P61026

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