| Description : |
GTP-hydrolysis activating protein (GAP) for small GTPase RAB22A, converting active RAB22A-GTP to the inactive form RAB22A-GDP. Plays a role in endocytosis and intracellular protein transport. Recruited by ANK2 to phosphatidylinositol 3-phosphate (PI3P)-positive early endosomes, where it inactivates RAB22A, and promotes polarized trafficking to the leading edge of the migrating cells. Part of the ANK2/RABGAP1L complex which is required for the polarized recycling of fibronectin receptor ITGA5 ITGB1 to the plasma membrane that enables continuous directional cell migration. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
92.3 kDa |
| AA Sequence : |
MEVRASLQKVSGSSDSVATMNSEEF VLVPQYADDNSTKHEEKPQLKIVSN GDEQLEKAMEEILRDSEKRPSSLLV DCQSSSEISDHSFGDIPASQTNKPS LQLILDPSNTEISTPRPSSPGGLPE EDSVLFNKLTYLGCMKVSSPRNEVE ALRAMATMKSSSQYPFPVTLYVPNV PEGSVRIIDQSSNVEIASFPIYKVL FCARGHDGTTESNCFAFTESSHGSE EFQIHVFSCEIKEAVSRILYSFCTA FKRSSRQVSDVKDSVIPTPDSDVFT FSVSLEVKEDDGKGNFSPVPKDRDK FYFKLKQGIEKKVVITVQQLSNKEL AIERCFGMLLSPGRNVKNSDMHLLD MESMGKSYDGRAYVITGMWNPNAPV FLALNEETPKDKQVYMTVAVDMVVT EVVEPVRFLLETVVRVYPANERFWY FSRKTFTETFFMRLKQSEGKGHTNA GDAIYEVVSLQRESDKEEPVTPTSG GGPMSPQDDEAEEESDNELSSGTGD VSKDCPEKILYSWGELLGKWHSNLG ARPKGLSTLVKSGVPEALRAEVWQL LAGCHDNQAMLDRYRILITKDSAQE SVITRDIHRTFPAHDYFKDTGGDGQ ESLYKICKAYSVYDEDIGYCQGQSF LAAVLLLHMPEEQAFCVLVKIMYDY GLRDLYRNNFEDLHCKFYQLERLMQ EQLPDLHSHFSDLNLEAHMYASQWF LTLFTAKFPLCMVFHIIDLLLCEGL NIIFHVALALLKTSKEDLLQADFEG ALKFFRVQLPKRYRAEENARRLMEQ ACNIKVPTKKLKKYEKEYQTMRESQ LQQEDPMDRYKFVYLTRTRPLEQKL ISEEDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
