Recombinant Human RAD52

• Cat.No.: RAD52-135H
• Product Overview: The product was highly purified from E. coli over-expressing human Rad52 protein as a recombinant protein.

Specification

• Description: Human Rad52 protein plays a major role in genetic recombination and recombination repair by mediating strand-annealing reaction between complementary DNA strands. Rad52 functionally and physically interacts with Rad51, Dmc1, and RPA in recombination processes. In radiation damaged cells, Rad52 is phosphorylated at Tyr104 by c-Abl tyrosine kinase and forms nuclear foci.
• Source: E. coli
• Species: Human
• Form: 20 mM potassium phosphate pH 7.5, 200 mM KCl, 2 mM 2ME, 0.5 mM EDTA, 20% glycerol
• Purity: >90% as judged from SDS-PAGE analysis
• Applications: 1) Studies on homologous recombination in mammals including human 2) Studies on the interaction of Rad52 protein with various proteins 3) To be used as a standard for Western blotting
• Storage: Store at -70℃.

Gene Information

• Gene Name: RAD52 RAD52 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: RAD52
• Synonyms: RAD52; RAD52 homolog (S. cerevisiae); RAD52 (S. cerevisiae) homolog; DNA repair protein RAD52 homolog; recombination protein RAD52; rhabdomyosarcoma antigen MU-RMS-40.23
• Gene ID: 5893
• mRNA Refseq: NM_134424
• Protein Refseq: NP_602296
• MIM: 600392
• UniProt ID: P43351
• Chromosome Location: 12p13-p12.2
• Pathway: Assembly of the RAD51-ssDNA nucleoprotein complex, organism-specific biosystem; DNA Repair, organism-specific biosystem; Double-Strand Break Repair, organism-specific biosystem; Homologous DNA pairing and strand exchange, organism-specific biosystem; Homologous Recombination Repair, organism-specific biosystem; Homologous recombination, organism-specific biosystem; Homologous recombination, conserved biosystem
• Function: DNA binding; protein binding