Recombinant Human RAF1 Protein, GST-tagged

• Cat.No.: RAF1-1123H
• Product Overview: Recombinant Human RAF1 Protein with GST tag was expressed in E.coli.

Specification

• Description: RafRBD (Ras binding domain, amino acids 50-132) mediates interaction with membrane-anchored Ras necessary for activation of the kinase activity of Raf. The GST-Tag facilitates the protein`s application in typical GST pull-down assays.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: Liquid
• Molecular Mass: 42 kDa
• Purity: > 95 % (SDS-PAGE)
• Notes: For in vitro use only!
• Storage: Store at -80 centigrade. Avoid freeze/thaw cycles.
• Expiry: Shelf Life: 12 months
• Storage Buffer: 32 mM Tris-HCl pH 7.6, 200 mM NaCl and 5 mM MgCl2
• Shipping: Shipped on dry ice.
• Protein length: 50-132 a.a.

Gene Information

• Gene Name: RAF1 Raf-1 proto-oncogene, serine/threonine kinase [ Homo sapiens (human) ]
• Official Symbol: RAF1
• Synonyms: RAF1; Raf-1 proto-oncogene, serine/threonine kinase; NS5; CRAF; Raf-1; c-Raf; CMD1NN; RAF proto-oncogene serine/threonine-protein kinase; C-Raf proto-oncogene, serine/threonine kinase; Oncogene RAF1; proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1; EC 2.7.11.1
• Gene ID: 5894
• mRNA Refseq: NM_002880
• Protein Refseq: NP_002871
• MIM: 164760
• UniProt ID: P04049

Reviews

04/18/2020

They understand that each research project may have unique requirements, and they are more than willing to address these specific needs.

11/01/2017

The manufacturer's commitment to providing excellent technical support is truly impressive.

02/25/2016

Their prompt and insightful responses to inquiries and troubleshooting requests enable me to tackle experimental obstacles with confidence and efficiency.

Q&As

How is the RAF1 protein studied in a clinical context?

The RAF1 protein is studied through a combination of molecular biology techniques, such as immunohistochemistry, Western blotting, and genetic sequencing, in both clinical and research settings.

Is RAF1 protein expression routinely tested in cancer diagnostics?

RAF1 protein expression is not typically included in routine cancer diagnostics. Instead, mutations and expression levels of other genes, like BRAF, are often analyzed in cancer patients.

Are there any clinical trials focused on RAF1 protein for disease treatment or research?

Clinical trials involving RAF1 protein are ongoing for conditions like Noonan syndrome and certain cancers. These trials aim to develop new therapies and better understand its role in disease.

How does the RAF1 protein influence cardiac health, and what clinical implications does this have?

RAF1 mutations can affect cardiac development, leading to congenital heart defects and other cardiac issues in patients with Noonan syndrome. This has important clinical implications for cardiologists and genetic counselors.

Can RAF1 mutations be inherited, and if so, how does this affect patients and their families?

Yes, RAF1 mutations can be inherited, and they can lead to Noonan syndrome. This can have a significant impact on affected individuals and their families, necessitating specialized medical care and genetic counseling.