Recombinant Human RAF1 Protein, His-tagged
Cat.No. : | RAF1-19H |
Product Overview : | Human Ras Binding Domain (RBD) of Raf-1 Proto-Oncogene, Serine/Threonine Kinase (RAF1), (UniProt # P04049, amino acids 1-149), expressed with an N-terminal 6XHis and glutathione S-transferase (GST) dual tag in E. coli. The dual 6xHis-GST tag allows immobilization of Raf1 RBD Protein and RBD-Ras complexes on either Ni-NTA or glutathione agarose resin columns. |
- Specification
- Gene Information
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Description : | The Raf1 RBD protein binds specifically to GTP-bound Ras proteins. Ras proteins, like other small GTPases, regulate molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Ras binds specifically to the Ras-binding domain (RBD) of Raf1 to control downstream signaling cascades. The affinity between RBD and GDP-bound Ras is three orders of magnitude lower. Therefore, the RBD protein can be used to specifically precipitate active, GTP-bound Ras as well as to specifically block the activity of Ras in vitro and in vivo. |
Source : | E. coli |
Species : | Human |
Tag : | His |
Purity : | > 80% by Coomassie staining |
Applications : | Measurement of the GTP/GDP bound ratio of Ras in vitro. Quantitation of GTP-bound Ras from tissue and tissue culture cell lysates. RAS/RAF binding studies. Inhibitor screening for RAS/RAF interaction |
Notes : | This product is for laboratory research use only. |
Storage : | Storage is recommended at -20 centigrade for longer periods of time. |
Concentration : | 1.0 mg/mL by A280 |
Storage Buffer : | 20 mM HEPES, pH 7.5, 100 mM NaCl, and 20% Glycerol. |
Shipping : | Product requires shipping on ice packs. |
Gene Name : | RAF1 Raf-1 proto-oncogene, serine/threonine kinase [ Homo sapiens (human) ] |
Official Symbol : | RAF1 |
Synonyms : | RAF1; Raf-1 proto-oncogene, serine/threonine kinase; NS5; CRAF; Raf-1; c-Raf; CMD1NN; RAF proto-oncogene serine/threonine-protein kinase; C-Raf proto-oncogene, serine/threonine kinase; Oncogene RAF1; proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1; EC 2.7.11.1 |
Gene ID : | 5894 |
mRNA Refseq : | NM_002880 |
Protein Refseq : | NP_002871 |
MIM : | 164760 |
UniProt ID : | P04049 |
Products Types
◆ Recombinant Protein | ||
RAF1-06H | Recombinant Human RAF1 Protein (RBD, 50-140), N-6×His and C-FLAG | +Inquiry |
RAF1-1123H | Recombinant Human RAF1 Protein, GST-tagged | +Inquiry |
Raf1-456M | Recombinant Mouse Raf1 Protein, MYC/DDK-tagged | +Inquiry |
RAF1-442H | Recombinant Human RAF1 Protein, His-tagged | +Inquiry |
RAF1-428H | Recombinant Human RAF1 Protein, His-tagged | +Inquiry |
◆ Lysates | ||
RAF1-1463HCL | Recombinant Human RAF1 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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