| Description : |
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
73.1 kDa |
| AA Sequence : |
MEHIQGAWKTISNGFGFKDAVFDGS SCISPTIVQQFGYQRRASDDGKLTD PSKTSNTIRVFLPNKQRTVVNVRNG MSLHDCLMKALKVRGLQPECCAVFR LLHEHKGKKARLDWNTDAASLIGEE LQVDFLDHVPLTTHNFARKTFLKLA FCDICQKFLLNGFRCQTCGYKFHEH CSTKVPTMCVDWSNIRQLLLFPNST IGDSGVPALPSLTMRRMRESVSRMP VSSQHRYSTPHAFTFNTSSPSSEGS LSQRQRSTSTPNVHMVSTTLPVDSR MIEDAIRSHSESASPSALSSSPNNL SPTGWSQPKTPVPAQRERAPVSGTQ EKNKIRPRGQRDSSYYWEIEASEVM LSTRIGSGSFGTVYKGKWHGDVAVK ILKVVDPTPEQFQAFRNEVAVLRKT RHVNILLFMGYMTKDNLAIVTQWCE GSSLYKHLHVQETKFQMFQLIDIAR QTAQGMDYLHAKNIIHRDMKSNNIF LHEGLTVKIGDFGLATVKSRWSGSQ QVEQPTGSVLWMAPEVIRMQDNNPF SFQSDVYSYGIVLYELMTGELPYSH INNRDQIIFMVGRGYASPDLSKLYK NCPKAMKRLVADCVKKVKEERPLFP QILSSIELLQHSLPKINRSASEPSL HRAAHTEDINACTLTTSPRLPVFTR TRPLEQKLISEEDLAANDILDYKDD DDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
