| Description : |
Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
99.2 kDa |
| AA Sequence : |
MVLRRMHRPRSCSYQLLLEHQRPSC IQGLRWTPLTNSEESLDFSESLEQA STERVLRAGRQLHRHLLATCPNLIR DRKYHLRLYRQCCSGRELVDGILAL GLGVHSRSQVVGICQVLLDEGALCH VKHDWAFQDRDAQFYRFPGPEPEPV GTHEMEEELAEAVALLSQRGPDALL TVALRKPPGQRTDEELDLIFEELLH IKAVAHLSNSVKRELAAVLLFEPHS KAGTVLFSQGDKGTSWYIIWKGSVN VVTHGKGLVTTLHEGDDFGQLALVN DAPRAATIILREDNCHFLRVDKQDF NRIIKDVEAKTMRLEEHGKVVLVLE RASQGAGPSRPPTPGRNRYTVMSGT PEKILELLLEAMGPDSSAHDPTETF LSDFLLTHRVFMPSAQLCAALLHHF HVEPAGGSEQERSTYVCNKRQQILR LVSQWVALYGSMLHTDPVATSFLQK LSDLVGRDTRLSNLLREQWPERRRC HRLENGCGNASPQMKARNLPVWLPN QDEPLPGSSCAIQVGDKVPYDICRP DHSVLTLQLPVTASVREVMAALAQE DGWTKGQVLVKVNSAGDAIGLQPDA RGVATSLGLNERLFVVNPQEVHELI PHPDQLGPTVGSAEGLDLVSAKDLA GQLTDHDWSLFNSIHQVELIHYVLG PQHLRDVTTANLERFMRRFNELQYW VATELCLCPVPGPRAQLLRKFIKLA AHLKEQKNLNSFFAVMFGLSNSAIS RLAHTWERLPHKVRKLYSALERLLD PSWNHRVYRLALAKLSPPVIPFMPL LLKDMTFIHEGNHTLVENLINFEKM RMMARAARMLHHCRSHNPVPLSPLR SRVSHLHEDSQVARISTCSEQSLST RSPASTWAYVQQLKVIDNQRELSRL SRELEPTRTRPLEQKLISEEDLAAN DILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
