Recombinant Human RBFOX1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: RBFOX1-853H
• Product Overview: A2BP1 MS Standard C13 and N15-labeled recombinant protein (NP_061193) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The Fox-1 family of RNA-binding proteins is evolutionarily conserved, and regulates tissue-specific alternative splicing in metazoa. Fox-1 recognizes a (U)GCAUG stretch in regulated exons or in flanking introns. The protein binds to the C-terminus of ataxin-2 and may contribute to the restricted pathology of spinocerebellar ataxia type 2 (SCA2). Ataxin-2 is the product of the SCA2 gene which causes familial neurodegenerative diseases. Fox-1 and ataxin-2 are both localized in the trans-Golgi network. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 42.6 kDa
• AA Sequence: MNCEREQLRGNQEAAAAPDTMAQPY ASAQFAPPQNGIPAEYTAPHPHPAP EYTGQTTVPEHTLNLYPPAQTHSEQ SPADTSAQTVSGTATQTDDAAPTDG QPQTQPSENTENKSQPKRLHVSNIP FRFRDPDLRQMFGQFGKILDVEIIF NERGSKGFGFVTFENSADADRAREK LHGTVVEGRKIEVNNATARVMTNKK TVNPYTNGWKLNPVVGAVYSPEFYA GTVLLCQANQEGSSMYSAPSSLVYT SAMPGFPYPAATAAAAYRGAHLRGR GRTVYNTFRAAAPPPPIPAYGGVVY QDGFYGADIYGGYAAYRYAQPTPAT AAAYSDSYGRVYAADPYHHALAPAP TYGVGAMNAFAPLTDAKTRSHADDV GLVLSSLQASIYRGGYNRFAPYTRT RPLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: RBFOX1 RNA binding fox-1 homolog 1 [ Homo sapiens (human) ]
• Official Symbol: RBFOX1
• Synonyms: RBFOX1; RNA binding protein, fox-1 homolog (C. elegans) 1; RNA binding protein fox-1 homolog 1; A2BP1; ataxin 2 binding protein 1; FOX 1; hexaribonucleotide binding protein 1; HRNBP1; fox-1 homolog A; ataxin 2-binding protein 1; hexaribonucleotide-binding protein 1; 2BP1; FOX1; FOX-1
• Gene ID: 54715
• mRNA Refseq: NM_018723
• Protein Refseq: NP_061193
• MIM: 605104
• UniProt ID: Q9NWB1