| Description : |
The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene. |
| Source : |
E. coli |
| Species : |
Human |
| Form : |
Solution |
| Molecular Mass : |
110 kDa |
| Protein Length : |
1068 |
| AA Sequence : |
MFEDKPHAEGAAVVAAAGEALQALC QELNLDEGSAAEALDDFTAIRGNYS LEGEVTHWLACSLYVACRKSIIPTV GKGIMEGNCVSLTRILRSAKLSLIQ FFSKMKKWMDMSNLPQEFRERIERL ERNFEVSTVIFKKYEPIFLDIFQNP YEEPPKLPRSRKQRRIPCSVKDLFN FCWTLFVYTKGNFRMIGDDLVNSYH LLLCCLDLIFANAIMCPNRQDLLNP SFKGLPSDFHTADFTASEEPPCIIA VLCELHDGLLVEAKGIKEHYFKPYI SKLFDRKILKGECLLDLSSFTDNSK AVNKEYEEYVLTVGDFDERIFLGAD AEEEIGTPRKFTRDTPLGKLTAQAN VEYNLQQHFEKKRSFAPSTPLTGRR YLREKEAVITPVASATQSVSRLQSI VAGLKNAPSDQLINIFESCVRNPVE NIMKILKGIGETFCQHYTQSTDEQP GSHIDFAVNRLKLAEILYYKILETV MVQETRRLHGMDMSVLLEQDIFHRS LMACCLEIVLFAYSSPRTFPWIIEV LNLQPFYFYKVIEVVIRSEEGLSRD MVKHLNSIEEQILESLAWSHDSALW EALQVSANKVPTCEEVIFPNNFETG NGGNVQGHLPLMPMSPLMHPRVKEV RTDSGSLRRDMQPLSPISVHERYSS PTAGSAKRRLFGEDPPKEMLMDKII TEGTKLKIAPSSSITAENVSILPGQ TLLTMATAPVTGTTGHKVTIPLHGV ANDAGEITLIPLSMNTNQESKVKSP VSLTAHSLIGASPKQTNLTKAQEVH STGINRPKRTGSLALFYRKVYHLAS VRLRDLCLKLDVSNELRRKIWTCFE FTLVHCPDLMKDRHLDQLLLCAFYI MAKVTKEERTFQEIMKSYRNQPQAN SHVYRSVLLKSIPREVVAYNKNIND DFEMIDCDLEDATKTPDCSSGPVKE ERGDLIKFYNTIYVGRVKSFALKYD LANQDHMMDAPPLSPFPHIKQQPGS PRRISQQHSIYISPHKNGSGLTPRS ALLYKFNGSPSKSLKDINNMIRQGE QRTKKRVIAIDSDAESPAKRVCQEN DDVLLKRLQDVVSERANH |
| Purity : |
> 90% |
| Applications : |
WB; ELISA; FACS; FC |
| Stability : |
This bioreagent is stable at 4 centigrade (short-term) and -70 centigrade(long-term). After reconstitution, sample may be stored at 4 centigrade for 2-7 days and below -18 centigrade for future use. |
| Storage : |
-70°C |
| Concentration : |
1 mg/mL |
| Storage Buffer : |
PBS (pH 7.4-7.5). Sterile-filtered colorless solution. |
| Reconstitution : |
Reconstitute in sterile distilled H2O to no less than 100 μg/mL; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance. |
