Active Recombinant Human REG4 protein, His-tagged

• Cat.No.: REG4-426H
• Product Overview: Active Recombinant Human REG4 protein(NP_001152824.1) (Met 1-Pro 158) was expressed in HEK293, with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-158 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by the ability of the immobilized protein to support the proliferation of HCT-116 human colorectal carcinoma cells (CCL-247) under low serum conditions. The ED50 for this effect is typically 4-20 μg/ml.
• Molecular Mass: The recombinant human REG4 consists of 147 amino acids and predictes a molecular mass of 17.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh REG4 is approximately 18 and 22 kDa due to different glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_001152824.1
• Unit ID: Q9BYZ8
• Unit ID-Weblink: https://www.uniprot.org/uniprotkb/Q9BYZ8

Gene Information

• Gene Name: REG4 regenerating islet-derived family, member 4 [ Homo sapiens ]
• Official Symbol: REG4
• Synonyms: REG4; regenerating islet-derived family, member 4; regenerating islet-derived protein 4; gastrointestinal secretory protein; GISP; REG IV; regenerating gene type IV; RELP; REG-4; reg IV; REG-like protein; regenerating islet-derived protein IV; REG-IV
• Gene ID: 83998
• mRNA Refseq: NM_001159352
• protein Refseq: NP_001152824
• MIM: 609846