| Species : |
Human |
| Source : |
E.coli |
| Tag : |
His |
| Description : |
RELM-beta (Resistin-Like Molecule-beta) is a member of a recently identified family of secreted proteins containing a conserved cystein-rich C-terminus. RELM-beta appears to be produced as a homodimer exclusively by intestinal goblet cells and can be found in high quantities in stool. Remarkably, stool of germ-free mice displaying sterile intestinal tract does not contain RELM-beta until bacterial colonization takes place after pathogen-free mice entered natural environment. High doses of recombinant RELM-beta showed hyperglycemic effects including lowered glucose disposal and increased hepatic glucose production in mice. |
| Amino acid sequence : |
MGSTQCSLDS VMDKKIKDVL NSLEYSPSPI SKKLSCASVK SQGRPSSCPA GMAVTGCACG YGCGSWDVQLETTCHCQCSV VDWTTARCCH LTKLRSHHHH HH. |
| Specificity : |
The amino acid sequence of the RELM-beta human recombinant is 100% homologous to the amino acid sequence of the RELM-beta human without signal sequence. |
| Purification Method : |
Three-step procedure using affinity Ni-NTA chromatography and size exclusionchromatography before and after refolding. |
| Purity : |
>95% (SDS-PAGE analyzed). |
| Formulation : |
Sterile filtered and lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH-4. |
| Reconstitution : |
Add 0.2 ml of 0.1 M Acetate buffer pH-4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. |
| Stability/Shelf Life : |
The lyophilized protein remains stable until the expiry date when stored at -20°C. |
| Applications : |
Western blotting. |
| Storage : |
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
