Recombinant Human REV1, His-tagged

• Cat.No.: REV1-175H
• Product Overview: REV1,51-256aa, Human, His tag, E.coli

Specification

• Description: REV1 contains a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. Two alternatively spliced transcript variants that encode different proteins have been found. Recombinant human REV1 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol
• Molecular Mass: 25.2 kDa (227aa)
• Protein length: 51-256aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGVAIYVNGY TDPSAEELRK LMMLHGGQYH VYYSRSKTTH IIATNLPNAK IKELKGEKVI RPEWIVESIK AGRLLSYIPY QLYTKQSSVQ KGLSFNPVCR PEDPLPGPSN IAKQLNNRVN HIVKKIETEN EVKVNGMNSW NEEDENNDFS FVDLEQTSPG RKQNGIPHPR GSTAIFNGHT PSSNGALKTQ DCLVPMVNSV ASRLSPA
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: REV1 REV1 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: REV1
• Synonyms: REV1; REV1 homolog (S. cerevisiae); REV1 (yeast homolog) like , REV1 like (yeast) , REV1L; DNA repair protein REV1; AIBP80; REV1- like; alpha integrin-binding protein 80; rev1-like terminal deoxycytidyl transferase; REV1L; FLJ21523; MGC26225; MGC163283
• Gene ID: 51455
• mRNA Refseq: NM_001037872
• Protein Refseq: NP_001032961
• MIM: 606134
• UniProt ID: Q9UBZ9
• Chromosome Location: 2q11.1-q11.2
• Pathway: DNA Damage Bypass, organism-specific biosystem; DNA Repair, organism-specific biosystem; Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem; Translesion synthesis by DNA polymerases bypassing lesion on DNA template, organism-specific biosystem; Translesion synthesis by HREV1, organism-specific biosystem
• Function: DNA-directed DNA polymerase activity; damaged DNA binding; deoxycytidyl transferase activity; magnesium ion binding; protein binding; transferase activity