Recombinant Human RFC1 Protein (402-492 aa), His-Myc-tagged
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Cat.No. : | RFC1-2319H |
Product Overview : | Recombinant Human RFC1 Protein (402-492 aa) is produced by E. coli expression system. This protein is fused with a 10xHis tag at the N-terminal and a Myc tag at the C-terminal. Research Area: Epigenetics and Nuclear Signaling. Protein Description: Partial. |
Description : | The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA. Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair. |
Source : | E. coli |
Species : | Human |
Tag : | His-Myc |
Form : | Tris-based buffer,50% glycerol |
Molecular Mass : | 14.8 kDa |
Protein length : | 402-492 aa |
AA Sequence : | GAENCLEGLI FVITGVLESI ERDEAKSLIE RYGGKVTGNV SKKTNYLVMG RDSGQSKSDK AAALGTKIID EDGLLNLIRT MPGKKSKYEI A |
Purity : | > 85% as determined by SDS-PAGE. |
Notes : | Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week. |
Storage : | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade. |
Concentration : | A hardcopy of COA will be sent along with the products. Please refer to it for detailed information. |
Figure : |
![]() (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. |
Gene Name : | RFC1 replication factor C (activator 1) 1, 145kDa [ Homo sapiens ] |
Official Symbol : | RFC1 |
Synonyms : | RFC1; A1; MHCBFB; PO GA; RFC140; A1 140 kDa subunit; RF-C 140 kDa subunit; RFC; PO-GA; RECC1; MGC51786; |
Gene ID : | 5981 |
mRNA Refseq : | NM_001204747 |
Protein Refseq : | NP_001191676 |
MIM : | 102579 |
UniProt ID : | P35251 |
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