Recombinant Human RFC1 Protein (402-492 aa), His-Myc-tagged

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Recombinant Human RFC1 Protein (402-492 aa), His-Myc-tagged

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Cat.No. : RFC1-2319H
Product Overview : Recombinant Human RFC1 Protein (402-492 aa) is produced by E. coli expression system. This protein is fused with a 10xHis tag at the N-terminal and a Myc tag at the C-terminal. Research Area: Epigenetics and Nuclear Signaling. Protein Description: Partial.
Description : The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA. Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.
Source : E. coli
Species : Human
Tag : His-Myc
Form : Tris-based buffer,50% glycerol
Molecular Mass : 14.8 kDa
Protein length : 402-492 aa
AA Sequence : GAENCLEGLI FVITGVLESI ERDEAKSLIE RYGGKVTGNV SKKTNYLVMG RDSGQSKSDK AAALGTKIID EDGLLNLIRT MPGKKSKYEI A
Purity : > 85% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA will be sent along with the products. Please refer to it for detailed information.
Figure :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Gene Name : RFC1 replication factor C (activator 1) 1, 145kDa [ Homo sapiens ]
Official Symbol : RFC1
Synonyms : RFC1; A1; MHCBFB; PO GA; RFC140; A1 140 kDa subunit; RF-C 140 kDa subunit; RFC; PO-GA; RECC1; MGC51786;
Gene ID : 5981
mRNA Refseq : NM_001204747
Protein Refseq : NP_001191676
MIM : 102579
UniProt ID : P35251

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