Recombinant Human RFC4 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: RFC4-2256H
• Product Overview: RFC4 MS Standard C13 and N15-labeled recombinant protein (NP_002907) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 39.7 kDa
• AA Sequence: MQAFLKGTSISTKPPLTKDRGVAAS AGSSGENKKAKPVPWVEKYRPKCVD EVAFQEEVVAVLKKSLEGADLPNLL FYGPPGTGKTSTILAAARELFGPEL FRLRVLELNASDERGIQVVREKVKN FAQLTVSGSRSDGKPCPPFKIVILD EADSMTSAAQAALRRTMEKESKTTR FCLICNYVSRIIEPLTSRCSKFRFK PLSDKIQQQRLLDIAKKENVKISDE GIAYLVKVSEGDLRKAITFLQSATR LTGGKEITEKVITDIAGVIPAEKID GVFAACQSGSFDKLEAVVKDLIDEG HAATQLVNQLHDVVVENNLSDKQKS IITEKLAEVDKCLADGADEHLQLIS LCATVMQQLSQNCTRTRPLEQKLIS EEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: RFC4 replication factor C subunit 4 [ Homo sapiens (human) ]
• Official Symbol: RFC4
• Synonyms: RFC4; replication factor C (activator 1) 4, 37kDa; replication factor C (activator 1) 4 (37kD); replication factor C subunit 4; A1; A1 37 kDa subunit; activator 1 37 kDa subunit; RFC 37 kDa subunit; RFC37; RF-C 37 kDa subunit; activator 1 subunit 4; replication factor C 37 kDa subunit; MGC27291
• Gene ID: 5984
• mRNA Refseq: NM_002916
• Protein Refseq: NP_002907
• MIM: 102577
• UniProt ID: P35249