Recombinant Human RGMA protein(Met1-Gly422), His-tagged

• Cat.No.: RGMA-7011H
• Product Overview: Recombinant Human RGMA isoform 3 (NP_064596.2) (Met 1-Gly 422), without the C-terminal pro peptide, was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-422 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant human RGMA consists of 386 amino acids and has a predicted molecular mass of 43 kDa. As a result of glycosylation and proteolytic processing, rhRGMA migrates mainly as an approximately 36 kDa in SDS-PAGE corresponding to the mature segment under reducing conditions, and the un-cleaved pro form can also be seen.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: RGMA RGM domain family, member A [ Homo sapiens ]
• Official Symbol: RGMA
• Synonyms: RGMA; RGM domain family, member A; repulsive guidance molecule A; RGM
• Gene ID: 56963
• mRNA Refseq: NM_001166283
• Protein Refseq: NP_001159755
• MIM: 607362
• UniProt ID: Q96B86