Recombinant Human ribosomal protein L11, His-tagged

• Cat.No.: RPL11-266H
• Product Overview: RPL11, 1-178aa, Human, His tag, E.coli

Specification

• Description: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL11 is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L5P family of ribosomal proteins. It is located in the cytoplasm. The protein probably associates with the 5S rRNA. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Recombinant human RPL11 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 1mM DTT, 2mM EDTA, 250mM Imidazole
• Molecular Mass: 22.6kDa (201aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAQDQGE KENPMRELRI RKLCLNICVG ESGDRLTRAA KVLEQLTGQT PVFSKARYTV RSFGIRRNEK IAVHCTVRGA KAEEILEKGL KVREYELRKN NFSDTGNFGF GIQEHIDLGI KYDPSIGIYG LDFYVVLGRP GFSIADKKRR TGCIGAKHRI SKEEAMRWFQ QKYDGIILPG K
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by BRADFORD assay)
• Protein length: 1-178

Gene Information

• Gene Name: RPL11 ribosomal protein L11 [ Homo sapiens ]
• Official Symbol: RPL11
• Synonyms: RPL11; ribosomal protein L11; 60S ribosomal protein L11; L11; CLL-associated antigen KW-12; cell growth-inhibiting protein 34; DBA7; GIG34
• Gene ID: 6135
• mRNA Refseq: NM_000975
• Protein Refseq: NP_000966
• MIM: 604175
• UniProt ID: P62913
• Chromosome Location: 1p36.1-p35
• Pathway: Cap-dependent Translation Initiation, organism-specific biosystem; Cytoplasmic Ribosomal Proteins, organism-specific biosystem; Disease, organism-specific biosystem; Eukaryotic Translation Elongation, organism-specific biosystem; Eukaryotic Translation Initiation, organism-specific biosystem; Eukaryotic Translation Termination, organism-specific biosystem; Formation of a pool of free 40S subunits, organism-specific biosystem
• Function: RNA binding; protein binding; rRNA binding; structural constituent of ribosome; structural constituent of ribosome