Recombinant Human Ribosomal Protein L8, His-tagged

• Cat.No.: RPL8-5549H
• Product Overview: Recombinant humanRPL8 protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by conventional chromatography, after refolding of the isolatedinclusion bodies in a renaturation buffer.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: RPL8 (60S ribosomal protein L8) belongs to the ribosomal protein L2Pfamily. Ribosomes, theorganelles that catalyze protein synthesis, consist of a small 40S subunitand a large 60S subunit. The protein belongs to the L2P family of ribosomalproteins. It is located in the cytoplasm and exists as a component of the 60S subunit where it isthought to play a role in aminoacyl-tRNA binding, specifically at theribosomal subunit interface.
• Form: Liquid.In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 50% glycerol, 300mM NaCl,2mM EDTA.
• Molecular Weight: 30.2kDa (277aa),confirmed by MALDI-TOF.
• Purity: > 90%by SDS - PAGE.
• Concentration: 0.25mg/ml (determinedby Bradford assay).
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAKVVFRDPYRFK KRTELFIAAE GIHTGQFVYC GKKAQLNIGN VLPVGTMPEG TIVCCLEEKP GDRGKLARASGNYATVISHN PETKKTRVKL PSGSKKVISS ANRAVVGVVA GGGRIDKPIL KAGRAYHKYK AKRNCWPRVRGVAMNPVEHP FGGGNHQHIG KPSTIRRDAP AGRKVGLIAA RRTGRLRGTK TVQEKEN
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• OfficialSymbol: RPL8
• Pathways: Cap-dependentTranslation Initiation; Cytoplasmic Ribosomal Proteins; DevelopmentalBiology; Diabetes pathways; Eukaryotic Translation Elongation; EukaryoticTranslation Initiation; Eukaryotic Translation Termination; Formation of apool of free 40S subunits; GTP hydrolysis and joining of the 60S ribosomalsubunit; Metabolism; Metabolism of mRNA; Nonsense Mediated Decay Independentof the Exon Junction Complex; Ribosome; TNF-alpha/NF-kB Signaling Pathway;Viral mRNA Translation

Gene Information

• Gene Name: RPL8 ribosomal protein L8 [ Homosapiens ]
• Synonyms: RPL8;ribosomal protein L8; L8; 60S ribosomal protein L8
• Gene ID: 6132
• mRNA Refseq: NM_000973
• Protein Refseq: NP_000964
• MIM: 604177
• UniProt ID: P62917
• Chromosome Location: 8q24.3
• Function: RNA binding; rRNAbinding; structural constituent of ribosome