Recombinant Human RIPK2, GST-His

• Cat.No.: RIPK2-711H
• Product Overview: Recombinant Human RIPK2, N-terminal fragment, amino acids M1-E299, N-terminal GST-HIS6 fusion protein with a Thrombin cleavage site, was expressed inSf9insect cells.MW = 63,815 Da.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 1-299 a.a.
• Description: This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-κB and inducer of apoptosis in response to various stimuli.
• Purification: GST-Affinity Chromatography.
• Product Identity: RIPK2 was confirmed as RIPK2 by mass spectroscopy LC-ESI-MS/MS.
• Storage Buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
• Concentration: 0.206 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
• Specific Activity: 11 pmol/μg×min.
• Storage: -80°C. Avoid repeated freeze-thaw cycles!

Gene Information

• Gene Name: RIPK2 receptor-interacting serine-threonine kinase 2 [ Homo sapiens ]
• Synonyms: RIPK2; receptor-interacting serine-threonine kinase 2; CARD-carrying kinase; CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase; growth-inhibiting gene 30; receptor interacting protein 2; receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP) kinase; CCK; RICK; RIP2; CARD3; GIG30; CARDIAK; EC 2.7.11.1
• Gene ID: 8767
• mRNA Refseq: NM_003821
• Protein Refseq: NP_003812
• MIM: 603455
• UniProt ID: O43353
• Chromosome Location: 8q21
• Pathway: NOD-like receptor signaling pathway; Neurotrophin signaling pathway; Signaling in Immune system; Signalling by NGF
• Function: ATP binding; CARD domain binding; LIM domain binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; signal transducer activity; transferase activity