Recombinant Human RND1, His-tagged

• Cat.No.: RND1-3600H
• Product Overview: Recombinant human RND1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography (1-200 aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: RND1, also known as Rho 6, is Rho-related GTPases form a distinct branch of the Rho family, since they differ from other Rho proteins in size, charge, and biochemical properties. It appears to be constitutively in the activated GTP-bound form and acts as negative regulators of actin assembly and of cell adhesion. It contributes to regulating the organization of the actin cytoskeleton in response to extracellular growth factors.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 30% glycerol 0.1M NaCl,1mM DTT
• Molecular Weight: 24.5 kDa (220aa), confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
• Purity: > 95% by SDS - PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MKERRAPQPV VARCKLVLVG DVQCGKTAML QVLAKDCYPE TYVPTVFENY TACLETEEQR VELSLWDTSG SPYYDNVRPL CYSDSDAVLL CFDISRPETV DSALKKWRTE ILDYCPSTRV LLIGCKTDLR TDLSTLMELS HQKQAPISYE QGCAIAKQLG AEIYLEGSAF TSEKSIHSIF RTASMLCLNK PSPLPQKSPV
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: RND1 Rho family GTPase 1 [ Homo sapiens ]
• Official Symbol: RND1
• Synonyms: ARHS; RHO6; RHOS; FLJ42294; RND1; rho-related GTP-binding protein Rho6; GTP-binding protein; ras homolog gene family, member; Rho family GTPase 1; Rho6; Rnd1
• Gene ID: 27289
• mRNA Refseq: NM_014470
• Protein Refseq: NP_055285
• MIM: 609038
• UniProt ID: Q92730
• Chromosome Location: 12q12
• Function: GTP binding; GTPase activity; nucleotide binding; protein binding; receptor binding