Recombinant Human ROBO1 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human ROBO1 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : ROBO1-4672H
Product Overview : ROBO1 MS Standard C13 and N15-labeled recombinant protein (NP_001139316) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 175.7 kDa
AA Sequence : MIAEPAHFYL FGLICLCSGS RLRQEDFPPR IVEHPSDLIV SKGEPATLNC KAEGRPTPTI EWYKGGERVE TDKDDPRSHR MLLPSGSLFF LRIVHGRKSR PDEGVYVCVA RNYLGEAVSH NASLEVAILR DDFRQNPSDV MVAVGEPAVM ECQPPRGHPE PTISWKKDGS PLDDKDERIT IRGGKLMITY TRKSDAGKYV CVGTNMVGER ESEVAELTVL ERPSFVKRPS NLAVTVDDSA EFKCEARGDP VPTVRWRKDD GELPKSRYEI RDDHTLKIRK VTAGDMGSYT CVAENMVGKA EASATLTVQV GSEPPHFVVK PRDQVVALGR TVTFQCEATG NPQPAIFWRR EGSQNLLFSY QPPQSSSRFS VSQTGDLTIT NVQRSDVGYY ICQTLNVAGS IITKAYLEVT DVIADRPPPV IRQGPVNQTV AVDGTFVLSC VATGSPVPTI LWRKDGVLVS TQDSRIKQLE NGVLQIRYAK LGDTGRYTCI ASTPSGEATW SAYIEVQEFG VPVQPPRPTD PNLIPSAPSK PEVTDVSRNT VTLSWQPNLN SGATPTSYII EAFSHASGSS WQTVAENVKT ETSAIKGLKP NAIYLFLVRA ANAYGISDPS QISDPVKTQD VLPTSQGVDH KQVQRELGNA VLHLHNPTVL SSSSIEVHWT VDQQSQYIQG YKILYRPSGA NHGESDWLVF EVRTPAKNSV VIPDLRKGVN YEIKARPFFN EFQGADSEIK FAKTLEEAPS APPQGVTVSK NDGNGTAILV SWQPPPEDTQ NGMVQEYKVW CLGNETRYHI NKTVDGSTFS VVIPFLVPGI RYSVEVAAST GAGSGVKSEP QFIQLDAHGN PVSPEDQVSL AQQISDVVKQ PAFIAGIGAA CWIILMVFSI WLYRHRKKRN GLTSTYAGIR KVTYQRGGEA VSSGGRPGLL NISEPAAQPW LADTWPNTGN NHNDCSISCC TAGNGNSDSN LTTYSRPADC IANYNNQLDN KQTNLMLPES TVYGDVDLSN KINEMKTFNS PNLKDGRFVN PSGQPTPYAT TQLIQSNLSN NMNNGSGDSG EKHWKPLGQQ KQEVAPVQYN IVEQNKLNKD YRANDTVPPT IPYNQSYDQN TGGSYNSSDR GSSTSGSQGH KKGARTPKVP KQGGMNWADL LPPPPAHPPP HSNSEEYNIS VDESYDQEMP CPVPPARMYL QQDELEEEED ERGPTPPVRG AASSPAAVSY SHQSTATLTP SPQEELQPML QDCPEETGHM QHQPDRRRQP VSPPPPPRPI SPPHTYGYIS GPLVSDMDTD APEEEEDEAD MEVAKMQTRR LLLRGLEQTP ASSVGDLESS VTGSMINGWG SASEEDNISS GRSSVSSSDG SFFTDADFAQ AVAAAAEYAG LKVARRQMQD AAGRRHFHAS QCPRPTSPVS TDSNMSAAVM QKTRPAKKLK HQPGHLRRET YTDDLPPPPV PPPAIKSPTA QSKTQLEVRP VVVPKLPSMD ARTDRSSDRK GSSYKGREVL DGRQVVDMRT NPGDPREAQE QQNDGKGRGN KAAKRDLPPA KTHLIQEDIL PYCRPTFPTS NNPRDPSSSS SMSSRGSGSR QREQANVGRR NIAEMQVLGG YERGEDNNEE LEETESTRTR PLEQKLISEE DLAANDILDY KDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : ROBO1 roundabout guidance receptor 1 [ Homo sapiens (human) ]
Official Symbol : ROBO1
Synonyms : ROBO1; roundabout, axon guidance receptor, homolog 1 (Drosophila); roundabout (axon guidance receptor, Drosophila) homolog 1; roundabout homolog 1; DUTT1; FLJ21882; SAX3; deleted in U twenty twenty; MGC131599; MGC133277;
Gene ID : 6091
mRNA Refseq : NM_001145844
Protein Refseq : NP_001139316
MIM : 602430
UniProt ID : Q9Y6N7

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