Recombinant Human ROR1 cell lysate
Cat.No. : | ROR1-001HCL |
Product Overview : | Human ROR1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Tag : | Non |
Gene Name : | ROR1 receptor tyrosine kinase-like orphan receptor 1 [ Homo sapiens ] |
Official Symbol : | ROR1 |
Synonyms : | ROR1; receptor tyrosine kinase-like orphan receptor 1; NTRKR1; tyrosine-protein kinase transmembrane receptor ROR1; neurotrophic tyrosine kinase receptor-related 1; neurotrophic tyrosine kinase, receptor-related 1; dJ537F10.1; MGC99659; |
Gene ID : | 4919 |
mRNA Refseq : | NM_001083592 |
Protein Refseq : | NP_001077061 |
MIM : | 602336 |
UniProt ID : | Q01973 |
Chromosome Location : | 1p32-p31 |
Pathway : | Nuclear Receptors, organism-specific biosystem; |
Function : | ATP binding; Wnt-protein binding; kinase activity; nucleotide binding; receptor activity; transmembrane receptor protein tyrosine kinase activity; |
Products Types
◆ Recombinant Protein | ||
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ROR1-512H | Recombinant Human ROR1 Protein, His-tagged | +Inquiry |
ROR1-425H | Recombinant Full Length Human ROR1 Protein, His-tagged | +Inquiry |
ROR1-1905H | Recombinant Human ROR1 Protein, His (Fc)-Avi-tagged | +Inquiry |
Ror1-01M | Active Recombinant Mouse Ror1 Protein (Gln30-Glu403), C-mFc-tagged | +Inquiry |
◆ Lysates | ||
ROR1-002HCL | Recombinant Human ROR1 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (3)
Write a reviewCustomer service promptly feedback my requests, technical staff timely solve the problems that arise.
Excellent after-sales service, solving many experimental problems.
Fast and timely shipment helped me to get satisfactory experimental results in short time.
Q&As (4)
Ask a questionROR1 is barely expressed in normal, differentiated and mature tissues, but is highly expressed in tumor tissues.
Inhibit the expression of ROR1 can reduce the proliferation and migration ability of tumor cells.
ROR1 is involved in the regulation of cell metabolism, proliferation, apoptosis, and differentiation.
ROR1 is involved in the study of breast, lung and ovarian cancers.
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