Recombinant Human RPE65 Over-expression Lysate, Myc/DDK-tagged

• Cat.No.: RPE65-01HCL
• Product Overview: Transient overexpression lysate of retinal pigment epithelium-specific protein 65kDa (RPE65) with a C-Myc/DDK tag was expressed in HEK293T.
  Components:
  1 vial of 100 μg gene specific transient over-expression cell lysate in RIPA buffer
  1 vial of 100 μg whole HEK293T cell lysate in RIPA buffer
  1 vial of 250 μL 2×SDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Specification

• Description: This gene encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated, membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT), the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins, with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa.
• Source: HEK293T
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 61.4 kDa
• Stability: Lysate samples are stable for 12 months from the date of receipt when stored at -80 centigrade.
• Storage: Upon receiving, store the sample at -80 centigrade. Also after dilution, the protein sample should be aliquoted and stored at -80 centigrade for long term storage. Avoid repeated freeze-thaw cycles.
• Dilutions: Lysate samples can be diluted with 2×SDS Sample Buffer provided.
• Shipping: Dry ice

Gene Information

• Gene Name: RPE65 retinoid isomerohydrolase RPE65 [ Homo sapiens (human) ]
• Official Symbol: RPE65
• Synonyms: RPE65; retinoid isomerohydrolase RPE65; p63; BCO3; LCA2; RP20; rd12; mRPE65; sRPE65; retinoid isomerohydrolase; BCO family, member 3
  RBP-binding membrane protein; RPE65, retinoid isomerohydrolase; all-trans-retinyl-palmitate hydrolase; lutein isomerase; meso-zeaxanthin isomerase; retinal pigment epithelium specific protein 65; retinal pigment epithelium-specific 65 kDa protein; retinal pigment epithelium-specific protein 65kDa; retinitis pigmentosa 20 (autosomal recessive); retinol isomerase; EC 3.1.1.64; EC 5.3.3.22
• Gene ID: 6121
• mRNA Refseq: NM_000329
• Protein Refseq: NP_000320
• MIM: 180069
• UniProt ID: Q16518