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Recombinant Human RSPO2 HEK293T cell lysate

Cat.No.: RSPO2-42HCL
Product Overview: Human RSPO2 was derived from Human HEK293T cell line.
Description: This gene encodes a member of the R-spondin family of proteins. These proteins are secreted ligands of leucine-rich repeat containing G protein-coupled receptors that enhance Wnt signaling through the inhibition of ubiquitin E3 ligases. A chromosomal translocation including this locus that results in the formation of a gene fusion has been identified in multiple human cancers. Alternative splicing results in multiple transcript variants.
Source: HEK293T
Species: Human
Tag: C-terminal Myc/DDK
Form: The cell lysate is provided in RIPA buffer.
Molecular Mass: 28.1 kDa
Usage: 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
Storage: The lysate is shipped with dry ice. Upon receiving, store the sample at -20 centigrade. Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade. Avoid repeated freeze-thaw cycles.Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20 centigrade for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name: RSPO2 R-spondin 2 [ Homo sapiens ]
Official Symbol: RSPO2
Synonyms: RSPO2; R-spondin 2; R spondin 2 homolog (Xenopus laevis); R-spondin-2; MGC35555; R-spondin 2 homolog; roof plate-specific spondin-2; CRISTIN2; MGC43342;
Gene ID: 340419
mRNA Refseq: NM_178565
Protein Refseq: NP_848660
MIM: 610575
UniProt ID: Q6UXX9
Chromosome Location: 8q23.1
Function: heparin binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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