Recombinant Human RUBCN Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: RUBCN-6004H
• Product Overview: KIAA0226 MS Standard C13 and N15-labeled recombinant protein (NP_055502) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 108.4 kDa
• AA Sequence: MRPEGAGMELGGGEERLPEESRREH WQLLGNLKTTVEGLVSTNSPNVWSK YGGLERLCRDMQSILYHGLIRDQAC RRQTDYWQFVKDIRWLSPHSALHVE KFISVHENDQSSADGASERAVAELW LQHSLQYHCLSAQLRPLLGDRQYIR KFYTDAAFLLSDAHVTAMLQCLEAV EQNNPRLLAQIDASMFARKHESPLL VTKSQSLTALPSSTYTPPNSYAQHS YFGSFSSLHQSVPNNGSERRSTSFP LSGPPRKPQESRGHVSPAEDQTIQA PPVSVSALARDSPLTPNEMSSSTLT SPIEASWVSSQNDSPGDASEGPEYL AIGNLDPRGRTASCQSHSSNAESSS SNLFSSSSSQKPDSAASSLGDQEGG GESQLSSVLRRSSFSEGQTLTVTSG AKKSHIRSHSDTSIASRGAPESCND KAKLRGPLPYSGQSSEVSTPSSLYM EYEGGRYLCSGEGMFRRPSEGQSLI SYLSEQDFGSCADLEKENAHFSISE SLIAAIELMKCNMMSQCLEEEEVEE EDSDREIQELKQKIRLRRQQIRTKN LLPMYQEAEHGSFRVTSSSSQFSSR DSAQLSDSGSADEVDEFEIQDADIR RNTASSSKSFVSSQSFSHCFLHSTS AEAVAMGLLKQFEGMQLPAASELEW LVPEHDAPQKLLPIPDSLPISPDDG QHADIYKLRIRVRGNLEWAPPRPQI IFNVHPAPTRKIAVAKQNYRCAGCG IRTDPDYIKRLRYCEYLGKYFCQCC HENAQMAIPSRVLRKWDFSKYYVSN FSKDLLIKIWNDPLFNVQDINSALY RKVKLLNQVRLLRVQLCHMKNMFKT CRLAKELLDSFDTVPGHLTEDLHLY SLNDLTATRKGELGPRLAELTRAGA THVERCMLCQAKGFICEFCQNEDDI IFPFELHKCRTCEECKACYHKACFK SGSCPRCERLQARREALARQSLESY LSDYEEEPAEALALEAAVLEATTRT RPLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: RUBCN rubicon autophagy regulator [ Homo sapiens (human) ]
• Official Symbol: RUBCN
• Synonyms: RUBCN; rubicon autophagy regulator; SCAR15; RUBICON; KIAA0226; run domain Beclin-1-interacting and cysteine-rich domain-containing protein; RUN and cysteine rich domain containing beclin 1 interacting protein; RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein; baron; beclin-1 associated RUN domain containing protein; rundataxin
• Gene ID: 9711
• mRNA Refseq: NM_014687
• Protein Refseq: NP_055502
• MIM: 613516
• UniProt ID: Q92622