Active Recombinant Human S100B protein(Ser2-Glu92), hFc-tagged

• Cat.No.: S100B-68H
• Product Overview: Recombinant Human S100B (NP_006263.1) (Ser 2-Glu 92) was expressed in HEK293 with the fused Fc region of Human IgG1 at the N-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: N-hFc
• Protein length: Ser2-Glu92
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to bind mouse S100A1 in a functional ELISA. 2. Measured by its ability to bind TP53 in a functional ELISA. 3. Immobilized recombinant human Fc-S100B at 10 μg/mL (100 μl/well) can bind biotinylated human S100A1 with a linear range of 15.6-250 ng/mL.
• Molecular Mass: The recombinant human Fc/S100B is a disulfide-linked homodimeric protein. The reduced monomer consists of 328 amino acids and has a predicted molecular mass of 37.2 kDa. As a result of glycosylation, the apparent molecular mass of rhFc/S100B monomer is approximately 40 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: S100B S100 calcium binding protein B [ Homo sapiens ]
• Official Symbol: S100B
• Synonyms: S100B; S100 calcium binding protein B; S100 calcium binding protein, beta (neural); protein S100-B; S100beta; S-100 protein subunit beta; S-100 calcium-binding protein, beta chain; S100 calcium-binding protein, beta (neural); NEF; S100; S100-B
• Gene ID: 6285
• mRNA Refseq: NM_006272
• Protein Refseq: NP_006263
• MIM: 176990
• UniProt ID: P04271

Reviews

03/15/2022

One major advantage of S100B protein is its ability to contribute to the understanding of diseases related to aberrant cell cycle regulation, including cancer.

09/29/2019

The S100B protein demonstrates exceptional quality, making it an ideal candidate to fulfill my experimental requirements.

10/23/2018

The manufacturer of S100B protein plays a crucial role in supporting my research efforts.

Q&As

How does S100B help in the diagnosis of traumatic brain injury (TBI)?

Elevated S100B levels in the blood can indicate TBI, and its measurement can aid in TBI diagnosis and severity assessment.

What conditions can S100B be used to diagnose?

S100B can be used to diagnose brain injuries, such as traumatic brain injury and stroke.

How is S100B measured in clinical settings?

S100B levels are typically measured through a blood test.

What precautions should be taken when interpreting S100B results?

Results should be interpreted in the context of the patient's clinical history and other diagnostic tests.

How is S100B used in the context of neurodegenerative diseases?

S100B may help in monitoring disease progression and severity in conditions like Alzheimer's disease.