Active Recombinant Human SCARB2 Protein, Fc-tagged

• Cat.No.: SCARB2-0723H
• Product Overview: Human SCARB2, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Arg 27 - Thr 432 (Accession # AAH21892). This protein carries a human IgG1 Fc tag at the C-terminus. Predicted N-terminus: Arg 27

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 27-432 aa
• Description: Scavenger receptor class B member 2 (SCARB2) is also known as Lysosome membrane protein 2 (LIMP2), 85 kDa lysosomal membrane sialoglycoprotein (LGP85), CD36 antigen-like 2 (CD36L2, LIMP2), which belongs to the CD36 family. SCARB2 acts as a lysosomal receptor for glucosylceramidase (GBA) targeting.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human R-Spondin 1, His Tag at 5 μg/mL (100 μL/well) can bind Human SCARB2, Fc Tag with a linear range of 0.02-1.25 μg/mL.
• Molecular Mass: The protein has a calculated MW of 72.6 kDa. The protein migrates as 90-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU/μg by the LAL method/rFC method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: SCARB2 scavenger receptor class B member 2 [ Homo sapiens (human) ]
• Official Symbol: SCARB2
• Synonyms: SCARB2; scavenger receptor class B, member 2; CD36 antigen (collagen type I receptor, thrombospondin receptor) like 2 (lysosomal integral membrane protein II), CD36L2; lysosome membrane protein 2; HLGP85; LIMP 2; LIMPII; SR BII; LIMP II; CD36 antigen-like 2; lysosome membrane protein II; 85 kDa lysosomal membrane sialoglycoprotein; 85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2; CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2 (lysosomal integral membrane protein II); AMRF; EPM4; LGP85; CD36L2; LIMP-2; SR-BII
• Gene ID: 950
• mRNA Refseq: NM_001204255
• Protein Refseq: NP_001191184
• MIM: 602257
• UniProt ID: Q14108