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Recombinant Human SCD protein, GST-tagged

Cat.No.: SCD-28H
Product Overview: Recombinant Human SCD(1 a.a. - 100 a.a.) fused with GST tag at N-terminal was expressed in Wheat Germ.
Description: This gene encodes an enzyme involved in fatty acid biosynthesis, primarily the synthesis of oleic acid. The protein belongs to the fatty acid desaturase family and is an integral membrane protein located in the endoplasmic reticulum. Transcripts of approximately 3.9 and 5.2 kb, differing only by alternative polyadenlyation signals, have been detected. A gene encoding a similar enzyme is located on chromosome 4 and a pseudogene of this gene is located on chromosome 17.
Source: Wheat Germ
Species: Human
Tag : GST
Form: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Molecular Mass: 36.63 kDa
Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
Notes: Best use within three months from the date of receipt of this protein.
Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Gene Name: SCD stearoyl-CoA desaturase (delta-9-desaturase) [ Homo sapiens ]
Official Symbol: SCD
Synonyms: SCD; stearoyl-CoA desaturase (delta-9-desaturase); SCDOS, stearoyl CoA desaturase opposite strand; acyl-CoA desaturase; acyl CoA desaturase; delta 9 desaturase; FADS5; fatty acid desaturase; delta-9 desaturase; delta-9-desaturase; delta(9)-desaturase; predicted protein of HQ0998; stearoyl-CoA desaturase opposite strand; SCD1; SCDOS; MSTP008;
Gene ID: 6319
mRNA Refseq: NM_005063
Protein Refseq: NP_005054
MIM: 604031
UniProt ID: O00767
Chromosome Location: 10q23-q24
Pathway: Adipogenesis, organism-specific biosystem; Biosynthesis of unsaturated fatty acids, organism-specific biosystem; Biosynthesis of unsaturated fatty acids, conserved biosystem; Fatty Acid Biosynthesis, organism-specific biosystem; PPAR signaling pathway, organism-specific biosystem; PPAR signaling pathway, conserved biosystem; oleate biosynthesis II (animals), organism-specific biosystem;
Function: iron ion binding; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water; stearoyl-CoA 9-desaturase activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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