Recombinant Human SCRIB Protein, Myc/DDK-tagged

• Cat.No.: SCRIB-01HL
• Product Overview: Transient overexpression lysate of scribbled homolog (Drosophila) (SCRIB), transcript variant 1 with a C-Myc/DDK tag was expressed in HEK293T.
  Components:
  1 vial of 100 μg gene specific transient over-expression cell lysate in RIPA buffer
  1 vial of 100 μg whole HEK293T cell lysate in RIPA buffer
  1 vial of 250 μL 2×SDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Specification

• Description: This gene encodes a protein that was identified as being similar to the Drosophila scribble protein. The mammalian protein is involved in tumor suppression pathways. As a scaffold protein involved in cell polarization processes, this protein binds to many other proteins. The encoded protein binds to papillomavirus E6 protein via its PDZ domain and the C-terminus of E6. Two alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
• Source: HEK293T
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 177.6 kDa
• Stability: Avoid repeated freeze-thaw cycles. Lysate samples are stable for 12 months from the date of receipt when stored at -80 centigrade.
• Storage: Upon receiving, store the sample at -80 centigrade. Also after dilution, the protein sample should be aliquoted and stored at -80 centigrade for long term storage.
• Reconstitution: Lysate samples can be diluted with 2×SDS Sample Buffer provided.
• Shipping: The lysate is shipped with dry ice.

Gene Information

• Gene Name: SCRIB scribble planar cell polarity protein [ Homo sapiens (human) ]
• Official Symbol: SCRIB
• Synonyms: SCRIB; scribble planar cell polarity protein; CRIB1; SCRB1; SCRIB1; Vartul; protein scribble homolog; scribbled homolog; scribbled planar cell polarity protein
• Gene ID: 23513
• mRNA Refseq: NM_182706
• Protein Refseq: NP_874365
• MIM: 607733
• UniProt ID: Q14160