Recombinant Human SELPLG protein, His&hFc-tagged

• Cat.No.: SELPLG-834H
• Product Overview: Recombinant Human SELPLG protein(Met1-Val295)(AAC50061.1) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: 1-295 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Molecular Mass: The recombinant human PSGL-1/Fc is a disulfide-linked homodimer after removal of the signal peptide and the prppeptide. The reduced monomer consists of 526 amino acids and has a predicted molecular mass of 57.1 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh PSGL-1/Fc monomer is approximately 110-120 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SELPLG selectin P ligand [ Homo sapiens ]
• Official Symbol: SELPLG
• Synonyms: SELPLG; selectin P ligand; P-selectin glycoprotein ligand 1; CD162; PSGL 1; cutaneous lymphocyte-associated associated antigen; CLA; PSGL1; PSGL-1
• Gene ID: 6404
• mRNA Refseq: NM_001206609
• Protein Refseq: NP_001193538
• MIM: 600738
• UniProt ID: Q14242