Recombinant Human SEMA3B Protein, His-tagged

• Cat.No.: SEMA3B-2679H
• Product Overview: Recombinant Human SEMA3B Protein (Pro573-Trp749) with a N-His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene belongs to the class-3 semaphorin/collapsin family, whose members function in growth cone guidance during neuronal development. This family member inhibits axonal extension and has been shown to act as a tumor suppressor by inducing apoptosis. Alternative splicing of this gene results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 23.5 kDa
  Accurate Molecular Mass: 25 kDa
• Protein length: Pro573-Trp749
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: SEMA3B sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B [ Homo sapiens (human) ]
• Official Symbol: SEMA3B
• Synonyms: SEMA3B; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B; SEMAA; semaphorin-3B; LUCA 1; SemA; sema5; semaV; sema V; sema A(V); semaphorin A; semaphorin V; semaphorin-V; SEMA5; LUCA-1; FLJ34863
• Gene ID: 7869
• mRNA Refseq: NM_001005914
• Protein Refseq: NP_001005914
• MIM: 601281
• UniProt ID: Q13214