Recombinant Human SEMA4D Protein, 22-734aa, C-hIgG-His tagged

• Cat.No.: SEMA4D-04H
• Product Overview: Recombinant human semaphorin-4D (22-734aa), fused to hIgG-His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Specification

• Description: Semaphorin4D, also known as CD100, is a member of the Class 4 family of transmembrane immune and nervous system semaphorins. It is an important mediator of the movement and differentiation of multiple cell types, including those of the immune, vascular, and nervous systems. It serves important roles in T cell priming, antibody production, and cell-to-cell adhesion. Sema4D, produced by T cells, activated B cells and dendritic cells acts through a low affinity receptor termed CD72 in the immune system. Unligated CD72 inhibits antigen presenting cells that express it, and this inhibition is relieved by Sema4D binding. It is reflected in its ability to inhibit ovarian carcinoma cell survival.
• Source: HEK293
• Species: Human
• Tag: C-hIgG-His
• Protein length: 22-734aa
• Form: Liquid
• Molecular Mass: 106.1 kDa (952aa)
• AA Sequence: MAFAPIPRITWEHREVHLVQFHEPD IYNYSALLLSEDKDTLYIGAREAVF AVNALNISEKQHEVYWKVSEDKKAK CAEKGKSKQTECLNYIRVLQPLSAT SLYVCGTNAFQPACDHLNLTSFKFL GKNEDGKGRCPFDPAHSYTSVMVDG ELYSGTSYNFLGSEPIISRNSSHSP LRTEYAIPWLNEPSFVFADVIRKSP DSPDGEDDRVYFFFTEVSVEYEFVF RVLIPRIARVCKGDQGGLRTLQKKW TSFLKARLICSRPDSGLVFNVLRDV FVLRSPGLKVPVFYALFTPQLNNVG LSAVCAYNLSTAEEVFSHGKYMQST TVEQSHTKWVRYNGPVPKPRPGACI DSEARAANYTSSLNLPDKTLQFVKD HPLMDDSVTPIDNRPRLIKKDVNYT QIVVDRTQALDGTVYDVMFVSTDRG ALHKAISLEHAVHIIEETQLFQDFE PVQTLLLSSKKGNRFVYAGSNSGVV QAPLAFCGKHGTCEDCVLARDPYCA WSPPTATCVALHQTESPSRGLIQEM SGDASVCPDKSKGSYRQHFFKHGGT AELKCSQKSNLARVFWKFQNGVLKA ESPKYGLMGRKNLLIFNLSEGDSGV YQCLSEERVKNKTVFQVVAKHVLEV KVVPKPVVAPTLSVVQTEGSRIATK VLVASTQGSSPPTPAVQATSSGAIT LPPKPAPTGTSCEPKIVINTVPQLH SEKTMYLKSSDNR
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/mL (determined by absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• References: 1. Kumanogoh, A. and H. Kikutani (2004) Cell. Mol. Life Sci. 61:292-300.
  2. Janssen, B.J.C. et al. (2010) Nature 467:1118-1122.
  3. Kumanogoh, A. et al. (2005) Int. Immunol. 17:1277-1282.
  4. Ishida, I. et al. (2003) Int. Immunol. 15:1027-1034.

Gene Information

• Gene Name: SEMA4D sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D [ Homo sapiens (human) ]
• Official Symbol: SEMA4D
• Synonyms: SEMA4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; C9orf164, chromosome 9 open reading frame 164 , SEMAJ; semaphorin-4D; CD100; coll 4; FLJ39737; M sema G; A8; GR3; BB18; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D; SEMAJ; coll-4; C9orf164; M-sema-G; FLJ33485; FLJ34282; FLJ46484; MGC169138; MGC169141
• Gene ID: 10507
• mRNA Refseq: NM_006378
• Protein Refseq: NP_006369
• MIM: 601866
• UniProt ID: Q92854