Recombinant Human SEMA5A protein, hFc-tagged

• Cat.No.: SEMA5A-3894H
• Product Overview: Recombinant Human SEMA5A protein(Met 1-Thr 765)(NP_003957.2) was expressed in HEK293, fused with Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1-765 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Molecular Mass: The recombinant human SEMA5A (aa 1-765)/Fc is a disulfide-linked homodimer. The reduced monomer consists of 984 amino acids and predictes a molecular mass of 110.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh SEMA5A/Fc monomer is approximately 125-135 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SEMA5A sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A [ Homo sapiens ]
• Official Symbol: SEMA5A
• Synonyms: SEMA5A; sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A; SEMAF; semaphorin-5A; semF; sema F; semaphorin F; semaphorin-F; FLJ12815
• Gene ID: 9037
• mRNA Refseq: NM_003966
• Protein Refseq: NP_003957
• MIM: 609297
• UniProt ID: Q13591