Recombinant Human SENP8, His-tagged

• Cat.No.: SENP8-70H
• Product Overview: Recombinant human SENP8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-212 aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: SENP8 is a cysteine protease that is a member of the sentrin-specific protease family. This protein is involved in processing and deconjugation of the ubiquitin-like protein termed, neural precursor cell expressed developmentally downregulated 8(NEDD8).
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.
• Molecular Weight: 26.3 kDa (232aa), confirmed by MALDI-TOF
• Purity: > 95 % by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MDPVVLSYMD SLLRQSDVSL LDPPSWLNDH IIGFAFEYFA NSQFHDCSDH VSFISPEVTQ FIKCTSNPAE IAMFLEPLDL PNKRVVFLAI NDNSNQAAGG THWSLLVYLQ DKNSFFHYDS HSRSNSVHAK QVAEKLEAFL GRKGDKLAFV EEKAPAQQNS YDCGMYVICN TEALCQNFFR QQTESLLQLL TPAYITKKRG EWKDLITTLA KK
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°Cor -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: SENP8 SUMO/sentrin specific peptidase family member 8 [ Homo sapiens ]
• Official Symbol: SENP8
• Synonyms: SENP8; DEN1; NEDP1; PRSC2; SUMO/sentrin specific peptidase family member 8; sentrin-specific protease 8; deneddylase 1; deneddylase-1; OTTHUMP00000164640; protease, cysteine 2; NEDD8-specific protease 1; NEDD8 specific-protease cysteine 2; SUMO sentrin specific protease family member 8; protease, cysteine, 2 (NEDD8 specific); SUMO/sentrin specific protease family member 8; sentrin/SUMO-specific protease SENP8; Protease, cysteine 2; HsT175121; Deneddylase-1; Sentrin/SUMO-specific protease SENP83; EC 3.4.22
• Gene ID: 123228
• mRNA Refseq: NM_001166340
• Protein Refseq: NP_001159812
• MIM: 608659
• UniProt ID: Q96LD8
• Chromosome Location: 15q23
• Function: cysteine-type peptidase activity; peptidase activity; protein binding