Recombinant Human SERPINB4 protein, His-tagged

• Cat.No.: SERPINB4-1763H
• Product Overview: Recombinant Human SERPINB4 aa. (Arg144~Thr298) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a member of the serpin family of serine protease inhibitors. The encoded protein is highly expressed in many tumor cells and can inactivate granzyme M, an enzyme that kills tumor cells. This protein, along with serpin B3, can be processed into smaller fragments that aggregate to form an autoantigen in psoriasis, probably by causing chronic inflammation.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 22kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Arg144~Thr298
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Isoelectric Point: 6.8

Gene Information

• Gene Name: SERPINB4 serpin family B member 4 [ Homo sapiens (human) ]
• Official Symbol: SERPINB4
• Synonyms: SERPINB4; serpin family B member 4; PI11; SCCA1; SCCA2; LEUPIN; SCCA-2; serpin B4; SCCA2/SCCA1 fusion protein; peptidase inhibitor 11; protease inhibitor (leucine-serpin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 4; serpin peptidase inhibitor, clade B (ovalbumin), member 4; squamous cell carcinoma antigen 1; squamous cell carcinoma antigen 2
• Gene ID: 6318
• mRNA Refseq: NM_002974.3
• Protein Refseq: NP_002965.1
• UniProt ID: P48594