Recombinant Human SETD3 Protein, His-tagged

• Cat.No.: SETD3-048H
• Product Overview: Recombinant N-terminal His-tagged human SETD3 protein (2-594) was expressed in E. coli.

Specification

• Description: Protein-histidine N-methyltransferase that specifically mediates methylation of actin at 'His-73' (PubMed:30526847, PubMed:30626964, PubMed:30785395, PubMed:31388018, PubMed:31993215). Histidine methylation of actin is required for smooth muscle contraction of the laboring uterus during delivery (PubMed:30626964). Does not have protein-lysine N-methyltransferase activity and probably only catalyzes histidine methylation of actin (PubMed:30626964, PubMed:30785395, PubMed:31388018).
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: 69.2 kDa
• Protein length: 2-594
• Purity: ≥80% as determined by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme required to transfer one methyl group to actin peptide per minute using 8 μM actin peptide (LKYPIEHGIVTNWDDMEKIW-amide) at 37 centigrade in a Methyltransferase Colorimetric Assay Kit.
• Applications: WB, ELISA, and enzymatic assays
• Stability: ≥ 6 months
• Storage: At -80 centigrade.
• Storage Buffer: 50 mM HEPES, pH 8.0, with 150 mM sodium chloride and 10% glycerol

Gene Information

• Gene Name: SETD3 SET domain containing 3, actin histidine methyltransferase [ Homo sapiens (human) ]
• Official Symbol: SETD3
• Synonyms: SETD3; SET domain containing 3, actin histidine methyltransferase; hSETD3; C14orf154; actin-histidine N-methyltransferase; SET domain-containing protein 3; histone-lysine N-methyltransferase setd3; EC 2.1.1.85
• Gene ID: 84193
• mRNA Refseq: NM_032233
• Protein Refseq: NP_115609
• MIM: 615671
• UniProt ID: Q86TU7