| Description : |
This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
135.5 kDa |
| AA Sequence : |
MFLYNLTLQRATGISFAIHGNFSGT KQQEIVVSRGKILELLRPDPNTGKV HTLLTVEVFGVIRSLMAFRLTGGTK DYIVVGSDSGRIVILEYQPSKNMFE KIHQETFGKSGCRRIVPGQFLAVDP KGRAVMISAIEKQKLVYILNRDAAA RLTISSPLEAHKANTLVYHVVGVDV GFENPMFACLEMDYEEADNDPTGEA AANTQQTLTFYELDLGLNHVVRKYS EPLEEHGNFLITVPGGSDGPSGVLI CSENYITYKNFGDQPDIRCPIPRRR NDLDDPERGMIFVCSATHKTKSMFF SLAQTEQGDIFKITLETDEDMVTEI RLKYFDTVPVAAAMCVLKTGFLFVA SEFGNHYLYQIAHLGDDDEEPEFSS AMPLEEGDTFFFQPRPLKNLVLVDE LDSLSPILFCQIADLANEDTPQLYV ACGRGPRSSLRVLRHGLEVSEMAVS ELPGNPNAVWTVRRHIEDEFDAYII VSFVNATLVLSIGETVEEVTDSGFL GTTPTLSCSLLGDDALVQVYPDGIR HIRADKRVNEWKTPGKKTIVKCAVN QRQVVIALTGGELVYFEMDPSGQLN EYTERKEMSADVVCMSLANVPPGEQ RSRFLAVGLVDNTVRIISLDPSDCL QPLSMQALPAQPESLCIVEMGGTEK QDELGERGSIGFLYLNIGLQNGVLL RTVLDPVTGDLSDTRTRYLGSRPVK LFRVRMQGQEAVLAMSSRSWLSYSY QSRFHLTPLSYETLEFASGFASEQC PEGIVAISTNTLRILALEKLGAVFN QVAFPLQYTPRKFVIHPESNNLIII ETDHNAYTEATKAQRKQQMAEEMVE AAGEDERELAAEMAAAFLNENLPES IFGAPKAGNGQWASVIRVMNPIQGN TLDLVQLEQNEAAFSVAVCRFSNTG EDWYVLVGVAKDLILNPRSVAGGFV YTYKLVNNGEKLEFLHKTPVEEVPA AIAPFQGRVLIGVGKLLRVYDLGKK KLLRKCENKHIANYISGIQTIGHRV IVSDVQESFIWVRYKRNENQLIIFA DDTYPRWVTTASLLDYDTVAGADKF GNICVVRLPPNTNDEVDEDPTGNKA LWDRGLLNGASQKAEVIMNYHVGET VLSLQKTTLIPGGSESLVYTTLSGG IGILVPFTSHEDHDFFQHVEMHLRS EHPPLCGRDHLSFRSYYFPVKNVID GDLCEQFNSMEPNKQKNVSEELDRT PPEVSKKLEDIRTRYAFTRTRPLEQ KLISEEDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
