Recombinant Human SF3B3 Protein, MYC/DDK-tagged

• Cat.No.: SF3B3-2509H
• Product Overview: Recombinant protein of human splicing factor 3b, subunit 3 with a C-MYC/DDK tag was expressed in HEK293T.

Specification

• Description: This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq, Jul 2008].
• Source: HEK293 Cells
• Species: Human
• Tag: MYC/DDK
• Molecular Mass: 135.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Storage: Store at –80 centigrade. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: SF3B3 splicing factor 3b subunit 3 [ Homo sapiens (human) ]
• Official Symbol: SF3B3
• Synonyms: RSE1; SAP130; SF3b130; STAF130; SF3B3; splicing factor 3b subunit 3; splicing factor 3B subunit 3; SAP 130; pre-mRNA splicing factor SF3b, 130 kDa subunit; spliceosome-associated protein 130
• Gene ID: 23450
• mRNA Refseq: NM_012426
• Protein Refseq: NP_036558
• MIM: 605592
• UniProt ID: Q15393