Recombinant Human SFTPD protein(Met1-Phe375), His-tagged

• Cat.No.: SFTPD-3868H
• Product Overview: Recombinant Human SFTPD (NP_003010.4)(Met 1-Phe 375) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-Phe375
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The pro form of human SFTPD consists of 366 amino acids and predictes a molecular mass of 37 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of SFTPD is 47 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publication:
  Surfactant Proteins-A and -D Attenuate LPS-Induced Apoptosis in Primary Intestinal Epithelial Cells (IECs) (2018)

Gene Information

• Gene Name: SFTPD surfactant protein D [ Homo sapiens ]
• Official Symbol: SFTPD
• Synonyms: SFTPD; surfactant protein D; SFTP4, surfactant, pulmonary associated protein D; pulmonary surfactant-associated protein D; COLEC7; SP D; collectin 7; collectin-7; lung surfactant protein D; pulmonary surfactant apoprotein; surfactant, pulmonary-associated protein D; surfactant-associated protein, pulmonary 4; SP-D; PSP-D; SFTP4
• Gene ID: 6441
• mRNA Refseq: NM_003019
• Protein Refseq: NP_003010
• MIM: 178635
• UniProt ID: P35247