Recombinant Human SGSH Protein, His/GST-tagged

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Recombinant Human SGSH Protein, His/GST-tagged

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Cat.No. : SGSH-695H
Product Overview : Recombinant Human SGSH(Arg21~Asn389) fused with His/GST tag at N-terminal was expressed in E. coli.
Description : This gene encodes the enzyme sulfamidase; one of several enzymes involved in the lysosomal degradation of heparan sulfate. Mutations in this gene are associated with the lysosomal storage disease mucopolysaccaridosis IIIA, also known as Sanfilippo syndrome A, which results from impaired degradation of heparan sulfate. Transcripts of varying sizes have been reported but their biological validity has not been determined.
Source : E. coli
Species : Human
Tag : His/GST
Form : 100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Molecular Mass : 71.1kDa
Protein length : Arg21~Asn389
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : > 90%
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Reconstitution : Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Figure :

Gene Name : SGSH N-sulfoglucosamine sulfohydrolase [ Homo sapiens ]
Official Symbol : SGSH
Synonyms : SGSH; N-sulfoglucosamine sulfohydrolase; N-sulphoglucosamine sulphohydrolase; HSS; MPS3A; mucopolysaccharidosis type IIIA; SFMD; sulfamidase; sulphamidase; heparan sulfate sulfatase; sulfoglucosamine sulfamidase;
Gene ID : 6448
mRNA Refseq : NM_000199
Protein Refseq : NP_000190
MIM : 605270
UniProt ID : P51688

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