Recombinant human SHFM1, His-tagged

• Cat.No.: SHFM1-2440H
• Product Overview: Recombinant human SHFM1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: SHFM1, as known as 26S proteasome complex subunit DSS1, has been proposed to be a candidate gene for the autosomal dominant form of the heterogeneous limb developmental disorder split hand/split foot malformation type 1. In addition, it has been shown to directly interact with BRCA2. It also may play a role in the completion of the cell cycle.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl
• Molecular Mass: 10.7kDa (93aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSEKKQP VDLGLLEEDD EFEEFPAEDW AGLDEDEDAH VWEDNWDDDN VEDDFSNQLR AELEKHGYKMETS
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: SHFM1 split hand/foot malformation (ectrodactyly) type 1 [ Homo sapiens ]
• Official Symbol: SHFM1
• Synonyms: SHFM1; split hand/foot malformation (ectrodactyly) type 1; SHFD1; 26S proteasome complex subunit DSS1; deleted in split hand/foot 1; DSS1; ECD; SEM1; Shfdg1; SHSF1; split hand/foot deleted protein 1; deleted in split-hand/split-foot 1; deleted in split hand/split foot protein 1; split hand/foot malformation type 1 protein
• Gene ID: 7979
• mRNA Refseq: NM_006304
• Protein Refseq: NP_006295
• UniProt ID: P60896
• Chromosome Location: 7q21.3
• Pathway: Homologous recombination, organism-specific biosystem; Homologous recombination, conserved biosystem; Proteasome, organism-specific biosystem; Proteasome, conserved biosystem; Proteasome, 19S regulatory particle (PA700), organism-specific biosystem
• Function: peptidase activity; protein binding